FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11402008
Sequences flagged as poor quality0
Sequence length52-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT150600.010.71625120
AGTGTAG217750.010.304548120
AGAGTGT264850.09.4913845120
AGATCTC153600.09.452348120
GTGTAGA216700.09.357791120
GAGTGTA239350.09.32449120
AAGAGTG304650.09.058832120
TGTAGAT239000.08.911401120
GTAGGGA475900.08.383425120
TGTAGGG508550.08.317082120
GTGTAGG553900.08.210194120
GTAGATC230300.08.166971118-119
TCGTGTA634450.08.05671120
TAGGGAA455500.08.001573118-119
TCTCGGT141650.07.8779263120
CGTGTAG588900.07.722238120
GATCTCG181150.07.716727120
AGGGAAA517500.07.6051717120
GGGAAAG475650.07.3661556120
GTCGTGT676550.07.342534120
Produced by FastQC (version 0.11.5)