FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13271129
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163500.088.3068120
GAGCACA260000.053.51521120
AGAGCAC360750.040.97752120
AAGAGCA541250.026.726334118-119
GAAGAGC619400.023.225285120
GGAAGAG748600.019.23832116-117
CGGAAGA886250.016.326172116-117
TCGGAAG890300.016.146973114-115
CCGATCT86350.015.0803354
CGATCTA23450.015.059085
TCCGATC89950.014.9675243
ATCGGAA1027850.014.11338116-117
GATCGGA1055350.013.688057112-113
AGATCGG1154750.012.56564114-115
GATCTAG7652.4738256E-1012.2616476
TAGATCG311200.011.623847118-119
CTTCCGA121400.011.5129191
CTAGATC69600.011.167586114-115
CACGTCT51500.010.469602120
TTAGATC86900.09.976595118-119
Produced by FastQC (version 0.11.5)