FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14321126
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC115050.062.40017120
GAGCACA192150.039.16034120
AGAGCAC247000.032.329365120
AAGAGCA405650.019.732649120
GAAGAGC444550.019.157417120
GGAAGAG560900.015.514329120
TCGGAAG662250.013.594138120
CGGAAGA686800.012.754591118-119
GATCGGA725050.012.637308120
AGATCGG803750.011.925328120
CTAGATC48100.011.706232120
ATCGGAA762600.011.688055118-119
TAGATCG212950.011.177504120
TTAGATC79000.09.638277120
CCTAGAT21500.09.523378120
GAGATCG329400.09.265616120
AAGATCG304750.08.329579118-119
CAGATCG252300.07.887196120
GCTAGAT19750.07.1274652120
AGAGATC137300.06.7293572118-119
Produced by FastQC (version 0.11.5)