FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14209481
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC208700.0111.97924120
GAGCACA353150.066.7552120
AGAGCAC472750.049.56583118-119
AAGAGCA697200.033.258312118-119
GAAGAGC797200.029.382914116-117
GGAAGAG966300.023.955683116-117
CGGAAGA1115950.021.222939114-115
TCGGAAG1144750.020.230633114-115
ATCGGAA1317350.018.030802112-113
GATCGGA1365200.017.092611112-113
AGATCGG1500700.015.857116110-111
TCCGATC78500.015.5394363
CACGTCT61450.015.484475120
CCGATCT83600.014.4051834
TAGATCG331350.014.388035112-113
CACACGT52950.014.275333118-119
CTAGATC95500.013.737313110-111
ACACGTC60850.013.298891120
AAGATCG593950.012.945782110-111
GAGATCG505150.012.19335112-113
Produced by FastQC (version 0.11.5)