FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12918074
Sequences flagged as poor quality0
Sequence length55-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG23850.018.116827120
CCGATCT64300.016.5154744
TCCGATC73450.016.1735573
AAAAAAA1939400.014.066955120
CTTCCGA106400.011.5596421
CGTATCA886250.011.294782120
GCGTCGG77100.011.286302120
CCGTATC989100.011.254904120
CCTAGAA14600.011.0954021
CGATCTA18500.010.7021075
GTATCAT836350.010.598161120
GCCGTAT1077750.010.579706120
CGCCGTA1172500.010.34919120
TCATTAA698550.010.163079120
CATTAAA670600.010.094472120
ATCATTA777450.010.027086120
TATCATT875100.09.868274120
GGTCGCC1484300.09.784345120
CCTTCAT107950.09.615141
TGGTCGC1606650.09.53603120
Produced by FastQC (version 0.11.5)