FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12918074
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC256350.0128.79019120
GAGCACA415050.075.65539120
AGAGCAC555150.057.94475118-119
AAGAGCA808500.038.622787118-119
GAAGAGC895900.035.65578116-117
GGAAGAG1067200.028.844446116-117
CACGTCT82650.028.795622120
CACACGT82400.025.829277120
CGGAAGA1278350.025.086172114-115
ACACGTC89950.024.244076120
ACGTCTG98100.023.405533120
TCGGAAG1348750.022.88479114-115
CCGATCT69400.021.4249484
ATCGGAA1567400.020.767288112-113
GCACACG94600.020.236681118-119
TCCGATC78800.020.016633
CGTCTGA107700.019.372282120
GATCGGA1620650.019.307098112-113
CGATCTA21500.018.6912825
AGATCGG1736300.018.641169110-111
Produced by FastQC (version 0.11.5)