FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14859541
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC155300.053.284977120
GAGCACA236000.035.926014120
AGAGCAC301800.028.367014120
AAGAGCA464100.019.7752120
GAAGAGC504500.017.96485120
GGAAGAG585750.016.373526120
CGGAAGA690950.014.515287120
TCGGAAG690600.014.375393120
ATCGGAA832250.013.013108120
GATCGGA841000.012.855044120
AGATCGG976650.011.167142118-119
TAGATCG238850.010.75032120
CTAGATC60750.010.618899116-117
CACACGT45150.08.727655118-119
CCTAGAT22600.08.717923116-117
TTAGATC88250.08.426184116-117
CACGTCT52450.08.118851120
AAGATCG391400.08.102954118-119
ACACGTC57350.07.7576447120
CAGATCG372450.07.440186120
Produced by FastQC (version 0.11.5)