FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13119920
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153600.071.13211120
GAGCACA235150.046.818417120
AGAGCAC320300.034.914825120
AAGAGCA493850.023.335022120
GAAGAGC544050.020.894302118-119
GGAAGAG664250.017.474531120
CGGAAGA760300.015.148063118-119
TCGGAAG768100.014.944819116-117
ATCGGAA904300.012.824671116-117
GATCGGA912250.012.633743114-115
AGATCGG1000050.011.597584114-115
TAGATCG251000.011.166685116-117
CACACGT47000.09.988211118-119
CACGTCT50200.09.697842120
CTAGATC53650.09.39937110-111
ACACGTC51750.09.138593120
AAGATCG365100.09.09719112-113
GAGATCG339900.08.952769110-111
CAGATCG341550.08.736726112-113
TTAGATC89100.08.586939108-109
Produced by FastQC (version 0.11.5)