FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535896
Sequences flagged as poor quality0
Sequence length52-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT255850.09.336257120
GAGTGTA373350.08.873564120
AGATCTC249750.08.530962120
AGTGTAG348250.08.479088120
GTGTAGA347500.08.37649120
TCTCGGT210200.08.223087120
GGTGGTC164850.08.155191120
ATCTCGG237000.08.154224120
AAGAGTG461450.08.102863120
CTCGGTG205250.08.04127120
TGGTCGC137900.08.008085120
GTAGGGA675200.07.9910235120
TCGGTGG199600.07.9682045120
AGAGTGT408300.07.967004120
GTAGATC353250.07.7814126120
TGTAGGG720400.07.7729120
GGTCGCC150000.07.602168120
GTGTAGG782350.07.5486474120
GTGGTCG155250.07.226796118-119
TAGGGAA644950.07.2212234120
Produced by FastQC (version 0.11.5)