FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15042393
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC225400.0117.34801120
GAGCACA385850.068.55059120
AGAGCAC531950.049.851307118-119
AAGAGCA794100.033.49026118-119
GAAGAGC893250.029.627321116-117
GGAAGAG1085550.024.53592116-117
CGGAAGA1269900.020.921112114-115
TCGGAAG1322550.020.040794114-115
ACACGTC66850.019.045597120
CACGTCT65200.018.702467120
ATCGGAA1539950.017.35823112-113
CACACGT59150.017.05377118-119
GATCGGA1580400.016.891254112-113
AGATCGG1719650.015.590992110-111
TAGATCG421800.013.999074112-113
GAGATCG732050.013.24733110-111
CAGATCG628700.012.978979110-111
ACGTCTG79450.012.752407120
GCACACG78300.012.710681120
CTAGATC69650.012.230524110-111
Produced by FastQC (version 0.11.5)