FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13351284
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC176950.071.81617120
GAGCACA263000.047.692062120
AGAGCAC367050.037.297794120
AAGAGCA558850.023.698275118-119
GAAGAGC610900.021.887154118-119
GGAAGAG762300.018.499676120
CGGAAGA894400.015.767333120
TCGGAAG899000.015.648591118-119
CACGTCT41650.013.688761120
ATCGGAA1063800.013.237283118-119
GATCGGA1080450.013.06826116-117
AGATCGG1187300.011.805411116-117
CACACGT41650.010.967601118-119
TAGATCG273250.010.885107114-115
ACACGTC44800.010.196441118-119
CTAGATC62850.09.836536118-119
TTAGATC102650.09.569342114-115
AAGATCG453050.09.552026120
GAGATCG441150.09.1402235114-115
CAGATCG396200.08.599481114-115
Produced by FastQC (version 0.11.5)