FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14296540
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC155100.063.407135120
GAGCACA234050.040.460266120
AGAGCAC305750.031.79599118-119
AAGAGCA475650.022.113527120
GAAGAGC523250.019.287449118-119
GGAAGAG630850.016.91067120
TCGGAAG745900.014.634112120
CGGAAGA748650.014.388969120
ATCGGAA874500.012.86936120
GATCGGA910350.012.641574120
CACGTCT50200.011.935953120
AGATCGG1029250.011.25714120
CACACGT45750.011.225054118-119
TAGATCG223200.010.441128118-119
ACACGTC59500.09.632493120
CTAGATC56750.09.335586116-117
AAGATCG416400.08.711992120
GAGATCG407000.08.481144120
TTAGATC88200.08.033603118-119
CAGATCG408600.07.3799872120
Produced by FastQC (version 0.11.5)