FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11940209
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC120850.067.3371120
GAGCACA188150.043.99433120
AGAGCAC250350.033.196846120
AAGAGCA412100.021.07195120
GAAGAGC431750.019.665668118-119
GGAAGAG520700.016.47888118-119
CGGAAGA638400.014.4473120
TCGGAAG640350.014.091354118-119
GATCGGA771450.012.3267975120
ATCGGAA755750.012.164355118-119
CACGTCT54650.012.063542120
AGATCGG849950.011.0120535118-119
CACACGT55550.010.789202118-119
TAGATCG209650.010.45035116-117
ACACGTC59850.09.680213120
CTAGATC42200.09.152618116-117
ACGTCTG63400.09.033168118-119
TGAACTC64100.08.72672120
GAGATCG357400.08.55242118-119
CGTCTGA71000.08.16003118-119
Produced by FastQC (version 0.11.5)