FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13811228
Sequences flagged as poor quality0
Sequence length54-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2239250.013.862229120
GCGTCGG69650.011.62845120
TCTCGGG35150.09.558107120
CGTATCA890500.08.697642120
TCATTAA718400.08.417893120
CATTAAA688100.08.160814120
GTATCAT871550.07.9781165120
CGCCGTA1095050.07.905723120
GCCGTAT1045400.07.85078120
CCGTATC988000.07.7543097120
ATCATTA803850.07.717111120
GGTCGCC1296000.07.494645120
GTCGCCG1252250.07.483404120
TGGTCGC1371600.07.409607120
TCGCCGT1188150.07.2458587120
TATCATT908200.07.2267833120
GTGGTCG1463000.07.110727120
TTAAAAA777200.07.032258120
GGTGGTC1554650.06.977101120
ATTAAAA796950.06.9106803120
Produced by FastQC (version 0.11.5)