FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13171338
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC146850.069.62658120
GAGCACA218100.043.726944120
AGAGCAC293250.033.9646120
AAGAGCA457500.023.144072120
GAAGAGC505900.020.230652118-119
GGAAGAG628750.016.91405120
CGGAAGA747300.014.788393120
TCGGAAG743400.014.447329118-119
ATCGGAA888850.012.384249118-119
GATCGGA889500.012.234011116-117
AGATCGG974250.011.322368116-117
CTAGATC57750.010.983493116-117
CACGTCT44950.010.740703120
TAGATCG226950.010.044079116-117
AAGATCG441200.09.217852114-115
TTAGATC102300.08.881049114-115
CACACGT43750.08.616608120
GAGATCG336250.08.439605116-117
ACACGTC49400.08.09192118-119
CAGATCG315300.07.4496994118-119
Produced by FastQC (version 0.11.5)