FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13191440
Sequences flagged as poor quality0
Sequence length50-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA161600.010.766018120
AGTGTAG148400.09.86403120
AGAGTGT185150.09.753068120
TAGATCT104450.09.706814120
AAGAGTG215300.09.669052120
TAGGGAA316600.09.436629120
GTAGGGA337650.09.079305120
GTGTAGG420500.08.7742120
GTGTAGA162650.08.594099120
TGTAGGG391200.08.357879120
AAAGAGT267600.08.272536120
CGTGTAG446600.08.247991120
GTCGTGT514000.08.193556120
TCGTGTA476000.08.078851120
GTAGATC183250.07.9553766120
GGGAAAG351700.07.590775120
CGTCGTG607200.07.518849120
AGGGAAA370550.07.463672120
AGATCTC126750.07.4310493120
TGTAGAT195950.07.1948385120
Produced by FastQC (version 0.11.5)