FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12962131
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC127950.072.85541120
GAGCACA199100.045.63379120
AGAGCAC267300.034.518402118-119
AAGAGCA434950.022.985483120
GAAGAGC487250.020.572142120
GGAAGAG609200.016.701672120
TCGGAAG695200.014.517694118-119
CGGAAGA718100.014.178056118-119
ATCGGAA825050.013.381718120
GATCGGA823450.012.619084118-119
AGATCGG906950.011.742994118-119
TAGATCG218400.011.323917120
CTAGATC54150.010.297421120
AAGATCG371600.08.9240885116-117
GAGATCG314350.08.817047118-119
TTAGATC92550.08.542401108-109
CAGATCG293000.08.194481120
GCTAGAT23650.07.489469116-117
TAAGATC160550.07.2323813116-117
TGAGATC139450.06.868191120
Produced by FastQC (version 0.11.5)