FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12149979
Sequences flagged as poor quality0
Sequence length67-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1112800.015.666499120
GCGTCGG85550.010.378513120
ATCATTA470550.09.447261120
TATCATT544800.09.371120
TCATTAA410150.09.346556120
GTATCAT543400.09.317862120
CGTATCA557200.09.2162895120
CATTAAA398250.08.932898120
CCGTATC629150.08.925141120
CGCCGTA725550.08.822476120
GCCGTAT676500.08.708375120
TGGTCGC919450.08.671421120
TCGCCGT809300.08.480274120
GGTCGCC871850.08.415464120
GTGGTCG990150.08.276433120
GGTGGTC1064350.08.178552120
ATTAAAA449850.08.108291120
GTCGCCG859750.08.045454120
TCTCGGG30250.07.932829120
CGGTGGT1173000.07.5181813120
Produced by FastQC (version 0.11.5)