FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12149979
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC159950.087.23184120
GAGCACA253600.053.628086120
AGAGCAC337600.040.784657118-119
AAGAGCA488950.027.736797118-119
GAAGAGC552950.024.949547116-117
GGAAGAG675150.019.97387116-117
CGGAAGA789250.017.503933114-115
TCGGAAG797800.016.88872114-115
ATCGGAA931550.014.942325112-113
GATCGGA953800.014.252122112-113
AGATCGG1045850.013.257899110-111
TAGATCG277350.011.914468110-111
ACACGTC52300.011.580128120
CTAGATC52200.011.162685108-109
CAGATCG473800.010.291713110-111
CACACGT45800.010.210623120
CACGTCT51150.09.967103118-119
TTAGATC83150.09.911551114-115
GAGATCG366750.09.773207110-111
ATAGATC161800.09.287592108-109
Produced by FastQC (version 0.11.5)