FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18378267
Sequences flagged as poor quality0
Sequence length54-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA249250.08.256206120
AGAGTGT276950.07.6903934120
AGTGTAG231200.07.5773373120
TGTAGGG443000.07.448702120
TAGATCT185100.07.3900948120
TCGTGTA544050.07.068721120
GTCGTGT565400.06.8760777120
TAGGGAA406300.06.8663783120
CGTGTAG509450.06.853983120
GTAGATC247150.06.748477120
AAGAGTG323500.06.6208773120
GTAGGGA407500.06.4928083118-119
GTGTAGG485700.06.42328120
AGCGTCG694850.06.216745120
GTGTAGA252200.06.1613555120
CTAGATC64950.06.0042086120
CGTCGTG651250.05.992681118-119
AGAGCGT790250.05.8382597120
GCGTCGT663600.05.777182118-119
GAGCGTC747700.05.7131314120
Produced by FastQC (version 0.11.5)