FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18378267
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC120500.035.794415120
GAGCACA159700.027.393044120
AGAGCAC193450.024.487862120
GAAGAGC359750.014.790481120
AAGAGCA347600.013.928734120
GGAAGAG469100.012.32507120
TCGGAAG477550.012.152016118-119
CGGAAGA557600.010.75455120
AGATCGG605150.010.610075120
GATCGGA565100.010.32363116-117
TAGATCG146300.010.289327120
ATCGGAA600750.09.81333118-119
CTAGATC38400.08.960288118-119
TTAGATC70850.06.894336118-119
AAGATCG324900.06.410844120
GAGATCG316650.06.0636725118-119
CCTAGAT20053.6379788E-125.5159883118-119
Produced by FastQC (version 0.11.5)