FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18065227
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC185200.067.6237120
GAGCACA282750.046.727413120
AGAGCAC379550.034.030525120
AAGAGCA618800.022.203676120
GAAGAGC684900.020.408285120
GGAAGAG873300.017.073553120
TCGGAAG1037700.014.821143120
CGGAAGA1048200.014.034468120
GATCGGA1226100.013.010673120
ATCGGAA1240150.012.526129120
AGATCGG1343250.011.966947120
TAGATCG330850.011.334735120
CTAGATC79350.09.970848114-115
GAGATCG486500.09.744478120
AAGATCG495550.09.293932120
CAGATCG462200.08.75376120
TTAGATC135900.08.614417116-117
ATAGATC162900.07.5419946120
CCTAGAT27450.07.1471257114-115
GCTAGAT27950.07.1343403120
Produced by FastQC (version 0.11.5)