FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13435559
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC219100.046.672554120
GAGCACA289700.034.13654120
AGAGCAC290600.033.451664120
AAGAGCA446700.021.204315120
GAAGAGC469350.020.120237118-119
CACGTCT86400.018.700087120
ACGTCTG96600.016.655855120
ACACGTC94900.015.965386118-119
GGAAGAG595200.015.7023118-119
CACACGT100000.014.675829120
CGTCTGA106400.014.172698120
TCGGAAG680350.013.722545116-117
CGGAAGA708650.013.411572116-117
TCTGAAC115850.012.2611885120
GCACACG137850.011.769457120
GTCTGAA126450.011.659271120
ATCGGAA842000.011.537199120
GATCGGA971050.010.932922120
AGATCGG1047250.010.37109118-119
TAGATCG253150.010.177458110-111
Produced by FastQC (version 0.11.5)