FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13919040
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC179200.077.96822120
GAGCACA280100.050.303314120
AGAGCAC377250.037.533207120
AAGAGCA577450.025.410475120
GAAGAGC651350.022.154337120
GGAAGAG793250.018.405752118-119
CGGAAGA943450.015.324713116-117
TCGGAAG954450.015.249955116-117
CACGTCT52650.014.244691120
ATCGGAA1121300.013.290323120
GATCGGA1144950.012.86125114-115
CACACGT48400.012.625976120
AGATCGG1276800.011.590128118-119
ACACGTC56800.011.247789118-119
CTAGATC76400.010.634668112-113
TAGATCG302600.010.590933110-111
ACGTCTG63000.09.810184120
GAGATCG524550.09.631052118-119
TTAGATC108000.09.451987108-109
AAGATCG374650.09.138011116-117
Produced by FastQC (version 0.11.5)