FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14453215
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC137300.059.63998120
GAGCACA207950.040.691193120
AGAGCAC274800.032.50302120
AAGAGCA444150.021.154055120
GAAGAGC479150.019.051992120
GGAAGAG607050.015.749516120
TCGGAAG710350.013.691718120
CGGAAGA742700.013.155218120
GATCGGA831450.012.377531120
ATCGGAA843000.012.057231120
TAGATCG226800.011.484074120
AGATCGG942300.011.204616120
CTAGATC58050.09.903791118-119
TTAGATC94000.09.292098116-117
CACACGT43100.08.8436120
AAGATCG397550.08.357283120
GAGATCG360500.08.352726120
CACGTCT49950.07.885175120
ACACGTC51000.07.847395120
CAGATCG335600.07.8177767120
Produced by FastQC (version 0.11.5)