FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20789235
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2287550.012.111668120
GTATCAT899300.09.639332120
CGTATCA900000.09.351491120
GCCGTAT1117350.09.295891120
CCGTATC1020750.09.251632120
GCGTCGG193300.09.199095120
TGGTCGC1547900.08.7982120
CGCCGTA1213150.08.650947120
GGTCGCC1464850.08.452206120
GGTGGTC1797350.08.41605120
GTGGTCG1683800.08.352108120
TCGGTGG2090300.08.130356120
CGGTGGT1964900.08.034709120
TCGCCGT1389600.07.989091120
GTCGCCG1484450.07.980435120
CTCGGTG2237300.07.921055120
TCTCGGT2364750.07.727859120
TCTCGGG52650.07.6447215120
ATCTCGG2538000.07.482007120
CATTAAA797250.07.4371724120
Produced by FastQC (version 0.11.5)