FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20789235
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC298950.0107.94068120
GAGCACA514150.062.697674120
AGAGCAC703550.046.37955120
AAGAGCA1039900.031.694332120
GAAGAGC1181150.027.86672118-119
GGAAGAG1413100.023.116575116-117
CGGAAGA1649800.020.085548116-117
TCGGAAG1738100.018.827826114-115
ATCGGAA2000100.016.601149114-115
GATCGGA2104350.015.61906112-113
AGATCGG2294100.014.44682112-113
TAGATCG592450.013.351499110-111
CTAGATC143200.011.871037108-109
GAGATCG750850.011.708805112-113
AAGATCG728600.011.453513114-115
TTAGATC240200.011.016485108-109
CACACGT67400.010.969703120
CAGATCG756450.010.944239110-111
CACGTCT72300.010.339877120
ATAGATC236150.010.008923110-111
Produced by FastQC (version 0.11.5)