FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20837892
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC217350.039.397606120
GAGCACA284750.030.626667120
AGAGCAC284350.027.585964118-119
AAGAGCA485450.017.613432120
GAAGAGC502450.016.879244120
GGAAGAG673350.013.973823120
TCGGAAG728250.012.780691118-119
CACGTCT97250.012.7273120
CGGAAGA808450.011.537112120
GATCGGA954850.011.150445120
CACACGT105100.011.103621118-119
ACACGTC105600.010.943519120
CTAGATC66300.010.763034120
ATCGGAA906200.010.493706118-119
AGATCGG1037400.010.08663120
TAGATCG265750.09.528859120
ACGTCTG120350.08.657802120
AAGATCG488350.08.23717120
GCACACG143350.08.008834118-119
TTAGATC109500.07.892273118-119
Produced by FastQC (version 0.11.5)