FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23499603
Sequences flagged as poor quality0
Sequence length53-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1974150.012.96477120
CGTATCA857900.09.622898120
ATCATTA780150.08.959255120
TCATTAA681950.08.83294120
GTATCAT859450.08.816716120
TGGTCGC1403100.08.184222120
CCGTATC997450.08.138245120
GGTCGCC1356600.08.066723120
GGTGGTC1628800.08.033633120
GTGGTCG1509850.08.03076120
GCCGTAT1096900.08.029401120
CATTAAA704900.07.96659120
CGCCGTA1197850.07.9387283120
TATCATT910400.07.796086120
GTCGCCG1418300.07.631195120
TCGCCGT1359750.07.5450335120
TCGGTGG1870300.07.42936120
CGGTGGT1792000.07.365611120
CTCGGTG2031800.07.0366707120
TCTCGGT2128050.06.856553120
Produced by FastQC (version 0.11.5)