FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558464
Sequences flagged as poor quality0
Sequence length52-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1523450.014.034653120
GCGTCGG91750.09.415613120
TCATTAA516900.08.646531120
CGTATCA613500.08.605186120
CCGTATC688850.08.412878120
CATTAAA508550.08.2104645120
ATCATTA573800.08.123676120
CGCCGTA797300.08.103751120
TATCATT650800.08.00137120
GGTCGCC940600.07.998061120
GTATCAT613300.07.9623933118-119
GCCGTAT738600.07.8624554120
GTCGCCG931750.07.5975714120
TGGTCGC985800.07.5583143120
TCGCCGT883200.07.3291655120
GTGGTCG1046050.07.174589118-119
GGTGGTC1116500.07.001287120
CGGTGGT1210500.06.7648787120
ATTAAAA631300.06.4334664120
TCGGTGG1262050.06.412503120
Produced by FastQC (version 0.11.5)