FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524805
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC181100.094.694824120
GAGCACA303850.057.62244120
AGAGCAC402050.043.129448118-119
AAGAGCA607800.028.793901120
GAAGAGC698750.024.865261116-117
GGAAGAG859750.020.346739118-119
CGGAAGA994050.017.571438114-115
TCGGAAG1011200.017.231483120
ATCGGAA1166150.014.948286118-119
GATCGGA1204850.014.468591114-115
AGATCGG1322100.013.159255110-111
TAGATCG340600.012.447283112-113
CTAGATC75150.011.750706118-119
TTAGATC98700.011.348315118-119
GAGATCG551400.010.752909116-117
CAGATCG477150.09.686325112-113
CCTAGAT28600.09.623918120
GGCCTAG3250.0048873129.410247114-115
ATAGATC172900.09.330648108-109
AAGATCG340550.09.317044116-117
Produced by FastQC (version 0.11.5)