FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15590861
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2479600.014.980062120
GCGTCGG108700.010.892898120
TCTCGGG47050.010.602913120
TCATTAA856800.09.638601120
CGTATCA1131750.09.415968120
GTATCAT1090600.09.379956120
CATTAAA812400.09.292369120
ATCATTA958300.09.182191120
CCGTATC1262950.09.104069120
GCCGTAT1352050.08.677484120
TATCATT1087700.08.664503120
CGCCGTA1451600.08.583395120
TGGTCGC1869600.08.348914120
GTCGCCG1718200.08.154086120
ATTAAAA897800.08.147375120
TTAAAAA875050.08.12566120
GTGGTCG2011650.08.049185120
GGTCGCC1766750.07.977641120
TCGCCGT1597750.07.805775120
GGTGGTC2147450.07.428217120
Produced by FastQC (version 0.11.5)