FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16439920
Sequences flagged as poor quality0
Sequence length55-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG78550.013.977782120
AAAAAAA1277450.012.150269120
TGGTCGC945400.09.22746120
GTCGCCG836850.09.162555120
GCCGTAT688200.09.136503120
TCGCCGT793500.09.1111555120
GGTCGCC878400.09.070665120
CGTATCA600450.08.982892120
GGTGGTC1110750.08.928734120
CGCCGTA715250.08.849689120
CCGTATC669400.08.729847120
GTGGTCG1024150.08.7171135120
GTATCAT608850.08.543625120
TCGGTGG1305200.08.540869120
CCTAGGA7952.962583E-48.3015191
TCATTAA508250.08.21609120
CGGTGGT1218050.08.083079120
ATCATTA592200.08.064511120
CTCGGTG1387800.08.006588120
TCTCGGT1486700.07.627318120
Produced by FastQC (version 0.11.5)