FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12199918
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC138900.075.591545120
GAGCACA222750.047.806446120
AGAGCAC304650.036.312595120
AAGAGCA480600.023.371202120
GAAGAGC526450.021.105927118-119
GGAAGAG641950.017.37716118-119
TCGGAAG754100.015.155683120
CGGAAGA752500.014.833282116-117
GATCGGA887500.012.790768118-119
ATCGGAA891500.012.728556120
AGATCGG969150.011.604766118-119
TAGATCG208900.010.836226114-115
AAGATCG465200.09.64695112-113
GAGATCG292450.09.40859116-117
CTAGATC59250.09.324988110-111
TTAGATC92900.09.26527114-115
TAAGATC197200.08.736377120
CAGATCG266950.08.314412116-117
CAAGATC250900.07.9208446120
CCTAGAT20900.07.4603744110-111
Produced by FastQC (version 0.11.5)