FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533506
Sequences flagged as poor quality0
Sequence length66-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT267800.010.395124120
AGTGTAG380400.010.188596120
AGAGTGT452000.09.782534120
GCGTCGG99950.09.544137120
AAGAGTG513250.09.070997120
GAGTGTA404950.08.993104120
GTAGATC384650.08.8750105120
GTAGGGA798800.08.704979120
ATCTCGG247900.08.640005120
AGATCTC277000.08.46875120
TAGGGAA758800.08.389004120
TCTCGGT229550.08.363634120
GTGGTCG162000.08.18464120
GTGTAGA376200.08.181273120
TGTAGGG857350.08.047518120
CGTGTAG995100.07.958469120
TCGTGTA1056300.07.83248120
GTGTAGG932500.07.675628120
TGTAGAT410100.07.6073937120
AAAGAGT600950.07.58748120
Produced by FastQC (version 0.11.5)