FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533506
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164350.063.220844120
GAGCACA245000.045.214165120
AGAGCAC328950.033.187195120
AAGAGCA511750.022.599945120
GAAGAGC584500.020.589062120
GGAAGAG728550.016.905996120
TCGGAAG858350.014.768427120
CGGAAGA897750.014.005828120
ATCGGAA1037500.013.0043745120
GATCGGA1052650.012.737905120
AGATCGG1166650.011.581283120
TAGATCG259950.011.215471120
CTAGATC75400.010.901597120
TTAGATC103050.09.97065120
GAGATCG431500.09.301452120
AAGATCG386650.09.151348120
CAGATCG492250.08.603606118-119
CACACGT42550.08.602542120
TCTAGAT31750.08.494877118-119
ACTAGAT32400.08.324455120
Produced by FastQC (version 0.11.5)