FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16515587
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC228350.093.877120
GAGCACA383450.057.5795120
AGAGCAC526650.042.03795120
AAGAGCA792200.028.177757118-119
GAAGAGC882700.024.917215118-119
GGAAGAG1077400.020.732738116-117
TCCGATC84700.020.459583
CCGATCT81950.020.4029884
CGATCTA21100.017.5803745
CGGAAGA1289350.017.286573116-117
TCGGAAG1327750.016.961725114-115
ATCGGAA1545750.014.515926114-115
GATCGGA1565050.014.468474112-113
AGATCGG1696550.013.167044112-113
CACGTCT66000.012.474707120
TAGATCG425600.012.29338110-111
CTTCCGA143250.012.1150281
CACACGT59500.011.785341118-119
ACACGTC64850.011.647637120
CTAGATC96550.011.439188108-109
Produced by FastQC (version 0.11.5)