FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16253259
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC111550.043.501366120
GAGCACA156300.033.33171120
AGAGCAC200600.027.935516120
AAGAGCA346250.016.113306120
GAAGAGC392250.015.008635120
GGAAGAG488000.013.590709120
GATCGGA615700.012.682251120
TCGGAAG535800.012.562142120
CGGAAGA591950.011.797072120
AGATCGG691500.011.398929120
ATCGGAA647850.010.912234120
TAGATCG177250.010.770149120
CTAGATC41100.010.188572120
TTAGATC72150.09.217936120
AAGATCG333450.08.107371120
CCTAGAT18750.07.8822083118-119
GCTAGAT20550.07.791261120
GAGATCG325300.07.2125225120
CAGATCG303950.06.584571120
TCTAGAT21800.06.4969463118-119
Produced by FastQC (version 0.11.5)