FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13985161
Sequences flagged as poor quality0
Sequence length79-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA663650.011.67169120
TGGTCGC457500.08.813021120
GGTCGCC449100.08.216345120
GTGGTCG501200.08.020664120
AGATCTC832000.07.9758763120
CTCGGTG681900.07.883769120
GCGTCGG91550.07.864471120
GGTGGTC552100.07.835453120
CGTATCA305600.07.6373525120
GCCGTAT388450.07.5530143120
TCGGTGG642100.07.475391120
GATCTCG813800.07.468586120
TAGATCT856550.07.4047704118-119
CCGTATC382100.07.3016753120
TCTCGGT701000.07.2588387118-119
TCATTAA234500.07.228778118-119
ATCTCGG753300.07.176347120
CGGTGGT628150.07.144714120
CGCCGTA405300.07.1353784120
GTGTAGA1069250.07.059078120
Produced by FastQC (version 0.11.5)