FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13985161
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167650.072.87698120
GAGCACA256600.045.42451120
AGAGCAC341400.035.245495120
AAGAGCA518300.023.15722118-119
GAAGAGC585100.021.209526120
GGAAGAG724200.016.974873120
TCGGAAG846900.014.653081120
CGGAAGA869450.014.320818118-119
ATCGGAA998150.012.498448116-117
GATCGGA1007800.012.497231120
AGATCGG1110400.011.271916118-119
CACACGT51800.010.979712120
TAGATCG206700.010.675123118-119
CTAGATC55300.09.665642116-117
TTAGATC77750.09.386465112-113
CACGTCT55700.09.165551118-119
AAGATCG477600.09.010273120
ACCTAGA10351.4826535E-58.606877120
ACACGTC57650.08.558069120
GAGATCG391350.08.518733118-119
Produced by FastQC (version 0.11.5)