FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12587555
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152000.069.37584120
GAGCACA231350.046.098457120
AGAGCAC306850.034.610435118-119
AAGAGCA472150.022.806225118-119
GAAGAGC498500.021.128235116-117
GGAAGAG617900.017.113834116-117
CACACGT43900.015.243654120
TCGGAAG696700.014.94116114-115
CGGAAGA722750.014.611936114-115
ACACGTC47850.013.396442120
ATCGGAA840500.012.790828112-113
GATCGGA842850.012.625777112-113
AGATCGG927200.011.590826110-111
TAGATCG199000.011.150325120
CACGTCT47350.010.860077120
CTAGATC52500.09.850109112-113
AAGATCG404200.09.12098110-111
ACGTCTG64900.09.008727120
GAGATCG326850.08.674872110-111
TTAGATC84650.08.602279118-119
Produced by FastQC (version 0.11.5)