FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12108670
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC168750.070.58251120
GAGCACA259800.046.689663120
AGAGCAC350850.035.62728120
AAGAGCA527300.023.672997118-119
GAAGAGC581850.021.212168120
GGAAGAG722700.017.608768120
CACGTCT59150.016.21118120
ACACGTC59000.015.555862120
CACACGT56500.015.15315120
CGGAAGA855950.014.8836118-119
TCGGAAG862600.014.435369118-119
ATCGGAA1037500.012.496943120
GATCGGA1051900.012.036412116-117
AGATCGG1157100.011.253311114-115
TAGATCG269850.010.952212118-119
ACGTCTG76500.010.743879120
GCACACG76550.09.842124120
CTAGATC69700.09.532528114-115
TTAGATC104400.09.250615116-117
AAGATCG469000.09.011221112-113
Produced by FastQC (version 0.11.5)