FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649600
Sequences flagged as poor quality0
Sequence length80-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1342950.013.485315120
GCGTCGG107050.011.267073120
TCTCGGG39300.09.924817120
CGCCGTA750050.09.136453120
CGTATCA573100.08.99424120
CCGTATC643100.08.836356120
GTATCAT568000.08.779189120
GCCGTAT691850.08.742796120
TCATTAA471900.08.53246120
GTGGTCG1030400.08.479246120
TGGTCGC952300.08.431096120
TCGCCGT841500.08.428787120
GGTCGCC902100.08.427982120
TCGGTGG1289400.08.153577120
CATTAAA471250.08.04762120
GTCGCCG891700.07.9531717118-119
CGGTGGT1206250.07.9246516120
GGTGGTC1106150.07.893156120
ATCATTA528900.07.873859120
CTCGGTG1379000.07.858782120
Produced by FastQC (version 0.11.5)