FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16454530
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC285450.0133.02461120
GAGCACA513500.075.34853120
AGAGCAC725600.052.87509118-119
AAGAGCA1039050.036.726166118-119
GAAGAGC1211450.031.686676116-117
GGAAGAG1458950.026.179188116-117
CGGAAGA1697750.022.657005114-115
TCGGAAG1813200.021.009104114-115
ATCGGAA2053650.018.720642112-113
GATCGGA2132850.017.809914112-113
AGATCGG2315900.016.56062110-111
CCTTCAT165200.015.2886761
CTAGATC81750.014.729166108-109
GAGATCG1064950.014.711365110-111
TAGATCG452750.014.709998110-111
CAGATCG724900.013.977424110-111
CCTTCAC111900.013.9747791
TTAGATC185450.013.576076108-109
CCTACAC41650.013.5474131
TGAGATC405500.013.367894108-109
Produced by FastQC (version 0.11.5)