FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11553473
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132150.063.316433120
GAGCACA208550.041.52779120
AGAGCAC271500.031.559216118-119
AAGAGCA406550.021.466713120
GAAGAGC460000.018.460651116-117
GGAAGAG549250.016.727036120
TCGGAAG624150.014.490363118-119
CGGAAGA637600.014.37786120
ATCGGAA752900.012.180377118-119
GATCGGA779350.011.933656116-117
AGATCGG872500.010.621583120
CACACGT41550.010.42995120
TAGGGGG15600.010.3684726
TAGATCG157550.010.011153116-117
CTAGATC41100.09.733057120
ACACGTC52700.08.85579120
CACGTCT47550.08.833441120
TTAGATC50150.08.360338116-117
CAGATCG411100.07.66518114-115
GAGATCG387100.07.6332536114-115
Produced by FastQC (version 0.11.5)