FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13583474
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC116200.069.958046120
GAGCACA178400.044.043835120
AGAGCAC234200.034.710186120
AAGAGCA380050.021.524876120
GAAGAGC407650.020.283697120
GGAAGAG504700.016.535192118-119
TCGGAAG593100.014.659528120
CGGAAGA601150.014.365498120
GATCGGA718000.013.060586120
ATCGGAA712950.012.813197120
AGATCGG802300.012.036082120
TAGATCG231550.010.84562118-119
TTAGATC109050.09.966261120
CTAGATC61450.09.499885118-119
AAGATCG328800.08.933557120
GAGATCG291500.08.640297118-119
CAGATCG265450.08.188516120
GCGCTAG5254.6801382E-57.8527713112-113
CACACGT35000.07.6576924118-119
CACGTCT36750.07.393338120
Produced by FastQC (version 0.11.5)