FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007984
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC156800.079.39423120
GAGCACA258700.048.713867120
AGAGCAC353300.035.48186118-119
AAGAGCA535300.023.652407118-119
GAAGAGC603050.021.013086120
GGAAGAG707450.017.793383116-117
CGGAAGA852450.014.819428114-115
TCGGAAG850900.014.756372114-115
GATCGGA990150.013.079432120
ATCGGAA995850.012.948605120
AGATCGG1089300.012.291824120
TAGATCG231200.011.510283120
CTAGATC46250.010.618377112-113
GAGATCG423550.010.2219715118-119
TTAGATC76550.09.873764118-119
CCTAGAT15300.09.56177120
AAGATCG348450.09.516224118-119
CAGATCG361600.09.314314114-115
ACACGTC43450.08.978602120
CACACGT38550.08.85487120
Produced by FastQC (version 0.11.5)