FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695704
Sequences flagged as poor quality0
Sequence length68-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT314800.09.8906145120
AGATCTC303750.09.657911120
TGGTCGC162700.09.033773120
GTGGTCG180900.08.987137120
TGTAGAT464400.08.88765120
ATCTCGG286900.08.865964120
GAGTGTA483100.08.767148120
AGTGTAG446900.08.6181755120
CTCGGTG255550.08.5920315120
GTGTAGA437250.08.575132120
GTAGATC429300.08.46842120
TCGGTGG238150.08.438881120
GATCTCG301850.08.426851120
AGAGTGT527500.08.302158120
AAGAGTG589050.08.3003435120
TCTCGGT264100.08.22301120
GGTGGTC200800.07.7529016118-119
CGGTGGT242550.07.420114120
GGTCGCC172450.07.375022120
TAGGGAA823150.07.258903120
Produced by FastQC (version 0.11.5)