FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695704
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136150.063.943375120
GAGCACA208200.041.7839120
AGAGCAC276550.032.675804120
AAGAGCA433850.020.70908120
GAAGAGC473450.019.30555120
GGAAGAG571300.015.987624118-119
TCGGAAG671150.014.893678120
CGGAAGA687000.013.549863120
GATCGGA785550.012.431756118-119
ATCGGAA793400.012.345521118-119
AGATCGG871300.011.30127120
TAGATCG226900.010.571132116-117
CTAGATC54700.010.25124112-113
TTAGATC92250.09.803069116-117
CACACGT43050.09.637047120
ACACGTC52000.09.224981120
GAGATCG308800.09.100515116-117
AAGATCG320100.08.9105425118-119
CCTAGAT20400.08.261998110-111
CAGATCG281250.07.997865120
Produced by FastQC (version 0.11.5)