FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15029679
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC175350.081.86952120
GAGCACA289600.047.78789120
AGAGCAC399950.037.761154120
AAGAGCA627300.024.531664120
GAAGAGC692250.022.053068118-119
GGAAGAG830450.018.396109120
CGGAAGA977000.015.743391116-117
TCGGAAG979550.015.460175116-117
ATCGGAA1164700.013.733911120
GATCGGA1165400.013.31515118-119
AGATCGG1280950.012.176692118-119
TAGATCG303050.011.652457116-117
CTAGATC85650.011.081943114-115
AAGATCG526700.010.679856120
TTAGATC130350.09.66379116-117
GAGATCG424200.09.608064120
CCTAGAT24650.08.918627114-115
CAGATCG374100.08.73097114-115
TAAGATC226400.08.384486118-119
TGAGATC196400.07.870912112-113
Produced by FastQC (version 0.11.5)